Figure 5.
Effect of tocilizumab (TCZ) treatment on the proliferation and steroid metabolism of 13914_1 cell line. Platinum-resistant 13914_1 cells, demonstrating a high endogenous IL-6 formation (302.6 pg/106 cells/h), were incubated with TCZ (250 µg/mL) for 72 h, followed by incubation with TCZ in the presence of increasing concentrations (0 to 2000 nM) of (A) DHEA and (B) E1 for 48 h and the viable cells were counted on a CASY® TT cell counter. Subsequently, the cellular supernatants of the samples containing 2000 nM of the steroid precursor (C) DHEA or (D) E1 were analyzed for steroid metabolites using LC-HRMS. Green indicates sensitivity for carboplatin and red represents carboplatin resistance. All data are presented as the means ± SD of three independent experiments. * p < 0.05.