Table 5.
Terpenes in hopped wort and beer brewed with Marynka (M) or Lubelski (L) hop pellets, fermented by L. thermotolerans (Lt) or S. cerevisiae (Sc).
| Compound [µg/L] | LRI 2 | Wort | Lt | Sc | Sig. 1 | |||
|---|---|---|---|---|---|---|---|---|
| L | M | L | M | L | M | |||
| Limonene | 1027 | 0.08 ab | 0.28 b | 0.00 a | 0.00 a | 0.00 a | 0.00 a | *** |
| cis-Linalol oxide | 1066 | 0.34 ab | 0.28 ab | 0.42 b | 0.34 ab | 0.41 b | 0.18 a | * |
| Linalool | 1092 | 1.21 | 0.80 | 0.98 | 0.80 | 1.15 | 0.44 | ns |
| Perillen 3 | 1101 | 0.31 b | 0.36 b | 0.00 a | 0.00 a | 0.00 a | 0.00 a | *** |
| α-Terpineol | 1171 | 0.84 | 0.69 | 0.70 | 0.68 | 0.46 | 0.65 | ns |
| Citronellol | 1210 | 0.00 a | 0.00 a | 0.88 ab | 2.22 c | 1.23 bc | 3.50 d | *** |
| Nerol 3 | 1218 | 0.59 b | 1.06 c | 0.00 a | 0.00 a | 0.00 a | 0.00 a | *** |
| Geraniol | 1257 | 3.41 c | 12.25 e | 1.28 b | 4.34 d | 0.60 ab | 0.34 a | *** |
| Perilla alcohol 3 | 1295 | 0.74 c | 0.41 b | 0.20 a | 0.09 a | 0.00 a | 0.00 a | *** |
| β-Damascenone | 1384 | 0.00 a | 0.00 a | 0.18 b | 0.16 b | 0.48 b | 0.40 b | * |
| β-Caryophyllene | 1414 | 1.06 bc | 1.33 c | 0.65 ab | 0.62 ab | 0.66 ab | 0.27 a | *** |
| Geranyl acetone 3 | 1443 | 0.83 ab | 1.09 b | 0.73 ab | 0.54 a | 0.65 ab | 0.51 a | * |
| Humulene | 1455 | 3.46 b | 3.61 b | 0.00 a | 0.00 a | 0.00 a | 0.00 a | *** |
| β-Farnesene 3 | 1462 | 2.43 b | 3.12 b | 0.70 a | 0.76 a | 0.57 a | 0.54 a | *** |
| α-Calacorene 3 | 1540 | 0.06 a | 0.08 ab | 0.13 bc | 0.14 c | 0.18 c | 0.13 bc | *** |
| Caryophyllene oxide | 1573 | 1.40 b | 1.85 c | 0.19 a | 0.20 a | 0.00 a | 0.00 a | *** |
| Nerolidol 3 | 1562 | 0.00 a | 0.00 a | 0.71 b | 0.63 b | 0.42 b | 0.41 b | *** |
| Humulol 3 | 1600 | 0.70 b | 0.71 b | 0.44 a | 0.31 a | 0.47 a | 0.31 a | * |
| Humulene epoxide II 3 | 1606 | 5.66 bc | 6.50 c | 3.66 ab | 2.61 a | 2.72 a | 1.80 a | *** |
| Patchulane 3 | 1618 | 2.78 b | 2.91 b | 1.65 a | 1.56 a | 1.71 a | 1.23 a | *** |
| Aromadendrene epoxide 3 | 1623 | 1.21 d | 1.05 cd | 0.71 bc | 0.53 b | 0.19 a | 0.13 a | *** |
| α-Muurolol 3 | 1632 | 0.51 b | 0.60 b | 0.24 a | 0.20 a | 0.17 a | 0.12 a | *** |
| τ-Muurolol 3 | 1646 | 1.69 d | 2.14 d | 0.89 c | 0.85 bc | 0.34 ab | 0.28 a | *** |
1 Sig.: significance; * and *** display the significance at 5% and 0.5% by least significant difference; ns: not significant. Values with different superscript roman letters (a-d) in the same raw are significantly different according to the Tukey’s range test (p < 0.05); 2 LRI—Linear Retention Index; 3 determined semi-quantitatively by measuring the relative peak area of each identified compound, according to the NIST [25] database, in relation to that of the internal standard.