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. 2020 Jan 23;12(2):280. doi: 10.3390/cancers12020280

Figure 3.

Figure 3

Glutathione synthesis is crucial for the survival and proliferation of SDHBKD cells. (A) Quantitative real-time PCR showed the knockdown efficiency of small interference ribonucleic acid (siRNA) targeting GCLC, GCLM, and SLC7A11 in hpheo1 cells. *** p < 0.001. (B) Glutathione quantification assay showed that the cellular GSH and GSSG level was decreased in SDHBKD hpheo1 cells with siRNA targeting GCLC, GCLM, and SLC7A11. *** p < 0.001. (C) CCK8 assay showed that the cell viability of SDHBKD hpheo1 cells was suppressed with siRNAs targeting GCLC, GCLM, or SLC7A11. (D) Direct cell count showed reduced cell number of SDHBKD hpheo1 cells with siRNAs targeting GCLC, GCLM, or SLC7A11. (E) Annexin V/PI apoptosis assay showed the apoptotic changes of SDHBKD hpheo1 cells with siRNAs targeting GCLC, GCLM, and SLC7A11. (F) Quantification of apoptosis assay, SDHBKD hpheo1 cells showed increased cell apoptosis. * p < 0.05; *** p < 0.001.