Table 3.
Optimum settings of temperature, ethanol concentration, and time that maximized both total monomeric catechins (mg/g DW) and total PACs (mg CE/g DW). The optimum (maximum) values were obtained by maximizing each response variable separately.
| Factor | Optimum Conditions for Total Monomeric Catechins | Optimum Conditions for Total PACs |
|---|---|---|
| Temperature | 170 °C | 120.3 °C |
| Ethanol concentration | 94% | 67.9% |
| Time | 55 min | 41.4 min |
| Predicted concentration under the optimum condition for each analyte | 18.3 mg/g DW | 113.6 mg CE/g DW |
| Predicted concentration of total monomeric catechins under the optimum condition of total PACs | 10.7 mg/g DW | n/a |
| Predicted concentration of total PACs under the optimum condition of total monomeric catechins | n/a | 43.7 mg CE/g DW |
Total monomeric catechins include catechin, epicatechin, and EGC; PACs: proanthocyanidins; CE: catechin equivalents; n/a: not applicable; DW, dry weight.