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. 2020 Jan 23;12(2):278. doi: 10.3390/cancers12020278

Figure 5.

Figure 5

Uptake of HA@TPCS2a-NPs in MDA-MB-231 and MCF-7 first generation mammospheres incubated for 2 or 24 h with 50 μg/mL HA@TPCS2a-NPs (TPCS2a dose 1.25 μg/mL). Column 1: Bright field images; Column 2: TPCS2a fluorescence at the equatorial plane of mammospheres; Column 3: Three-dimensional reconstruction of TPCS2a fluorescence distribution and intensity in the equatorial plane of the mammospheres; Column 4: Maximum projection obtained from the superimposition of 20 different acquired focal planes. Scale bar unit in the images is µm.