Figure 3.

Differentiation capacities of Fp, Fr, and F-DHJ fibroblasts into mesodermal lineages. Samples from 5 independent “young” donors (20, 22, 25, 28, and 31 years old) were tested for their capacity to differentiate into chondrocytes (toluidine blue and safranin O staining, aggrecan (ACAN) and collagen XIα1 (ColXIα1) expression, white arrows); adipocytes (presence of cytoplasmic lipid droplets, black arrows); and osteoblasts (alizarin-red staining). Scoring of differentiation capabilities are presented: (−) = not present, (+/−) = low representation, (++) = frequent representation, and (+++) = major representation.