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. 2020 Feb 5;9(2):368. doi: 10.3390/cells9020368

Table 2.

Antibodies. Antibody references and working dilutions are provided.

Protein name Supplier Reference Dilution
alpha Sm actin Sigma (Saint-Quentin Falaviers—France) A5228 1/200
ACAN (Aggrecan) * Abcam (Paris—France) ab3778 1/20
Col XI a1 Sigma (Saint-Quentin Falaviers—France) SAB4500393 1/50
Desmine (clone D33) Dako—Agilent (France) M0760 1/50
GAPDH Interchim for Meridian (France) H86504M 1/2000
KLF9 Abcam (Paris—France) ab170980 1/100 (IHC)–1/1000 (WB)
Phalloïdine Rhodamin Invitrogen (France) R415 1/50
TNC (Tenascin C) Novus Biologicals (Abington—UK) NB110-68136 1/50
Vimentin TEBU (Le Perray-en-Yvelines—France) MON3005 1/10
Goat anti-Mouse Alexa 488 Molecular Probes Invitrogen (France) A21121 1/250
Goat anti-Rabbit Alexa 555 Molecular Probes Invitrogen (France) A21428 1/250
Zenon Alexa 488 Molecular Probes Invitrogen (France) Z25002
Goat anti-Rabbit HRP Thermo-Fisher, France 32460 1/2000

* Pre-processing: keratanase (0.1 U/mL) + chondroitinase (0.1 U/mL)—3 h—37 °C.