Table 2.
Protein name | Supplier | Reference | Dilution |
---|---|---|---|
alpha Sm actin | Sigma (Saint-Quentin Falaviers—France) | A5228 | 1/200 |
ACAN (Aggrecan) * | Abcam (Paris—France) | ab3778 | 1/20 |
Col XI a1 | Sigma (Saint-Quentin Falaviers—France) | SAB4500393 | 1/50 |
Desmine (clone D33) | Dako—Agilent (France) | M0760 | 1/50 |
GAPDH | Interchim for Meridian (France) | H86504M | 1/2000 |
KLF9 | Abcam (Paris—France) | ab170980 | 1/100 (IHC)–1/1000 (WB) |
Phalloïdine Rhodamin | Invitrogen (France) | R415 | 1/50 |
TNC (Tenascin C) | Novus Biologicals (Abington—UK) | NB110-68136 | 1/50 |
Vimentin | TEBU (Le Perray-en-Yvelines—France) | MON3005 | 1/10 |
Goat anti-Mouse Alexa 488 | Molecular Probes Invitrogen (France) | A21121 | 1/250 |
Goat anti-Rabbit Alexa 555 | Molecular Probes Invitrogen (France) | A21428 | 1/250 |
Zenon Alexa 488 | Molecular Probes Invitrogen (France) | Z25002 | |
Goat anti-Rabbit HRP | Thermo-Fisher, France | 32460 | 1/2000 |
* Pre-processing: keratanase (0.1 U/mL) + chondroitinase (0.1 U/mL)—3 h—37 °C.