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. 2020 Feb 4;9(2):361. doi: 10.3390/cells9020361

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Image cytometry (Amnis) of the double staining: LysoTracker Red and curcumin. (a) Curcumin green fluorescence for 24-h incubation with 20 µM curcumin. (b) LysoTracker Red fluorescence analysis for 10-min incubation with 100 nM LysoTracker Red. (c) Fluorescence histogram of curcumin and LysoTracker green fluorescence. (d) A selection of 6 cells analyzed with Amnis where it is possible to see the widespread intracellular staining of 5 µM curcumin for 3 h and the punctate staining with LysoTracker Red.