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Detailed analysis (a–f) of the change in plasma membrane permeability of Huh-7 cells (using the xCELLigence device). YOPRO-1/PI staining was used and three cell populations could be distinguished: YO-PRO-1−/PI− for viable cells, YO-PRO-1+/PI− or YO-PRO-1+/PIintermediate for apoptotic cells and YO-PRO-1+/PI+++ for necrotic cells. The histograms are representatives of nine different experiments realized with the same flow cytometer setting.
