Figure 9.

Detection of the acidic vesicles by fluorescence and/or electron microscopy. (a)—Flow cytometry analysis of acridine orange (AO) staining of the acidic vesicles in samples treated with curcumin for 16 h. The histogram presents the fast increase in AO internalization in low pH vesicles. (b)—Image cytometry measurements presented in Figure 8. The sizes of the vesicles are given in µm and the number of vesicles is indicated. (c,d)—Electron microscopy picture of a sample of Huh-7 cells treated with 20 µM curcumin for 16 h. In (a), we can see essentially mitochondria (Mito.) with swollen ER (SER) and stress fibers (StF). In (b), essentially round, translucent mitochondria defective in cristae membranes with swollen ER (SER) and stress fibers (StF): in (e,f), essentially lysosomes (Lys) and autophagolysosomes (AutoP). With only one mitochondrion can be seen (Mito) in (f).