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Total anthocyanin contents in AtPAP1 overexpressed S. nigrum transgenic lines. Total anthocyanin was measured by spectrophotometry at A530 nm and A650 nm. All data are expressed as mean and SEM. All AtPAP1 overexpressed S. nigrum transgenic lines from first (T1) and second generation (T2) were compared with control and mock lines. The significance threshold was at a level of 5% (*** P < 0.001). All the experiments were biological triplicates.
