Table 2.
T cell phenotyping and activation markers.
| Fluorescence | Antibody | Clone | Isotype Control | Clone |
|---|---|---|---|---|
| BUV395 | anti-human CD3 | UCHT1 | - | |
| PE | anti-human CD4 | RPA-T4 | - | |
| BUV 737 | anti-human CD8 | SK1 | - | |
| BV 650 | anti-human CD279 (PD-1) | EH12.2H7 | mouse IgG1, κ | MOPC-21 |
| BV 711 | anti-human CD223 (LAG-3) | 11C3C65 | mouse IgG1, κ | MOPC-21 |
| PE/Dazzle594 | anti-human CD366 (Tim-3) | F38-2E2 | mouse IgG1, κ | MOPC-21 |
| PerCP-Cy5.5 | anti-human CD25 | BC96 | mouse IgG1, κ | P3.6.2.8.1 |
| PE-Cy7 | anti-human CD69 | FN50 | mouse IgG1, κ | MOPC-21 |
Abbreviations: BUV: Brilliant Ultraviolet, BV: Brilliant Violet, Cy: cyanine, IgG: immunoglobulin G, LAG-3: lymphocyte activation gene 3, PD-1: programmed cell death 1, PE: phycoerythrin, PerCP: peridinin-chlorophyll-protein complex, Tim-3: T cell immunoglobulin and mucin domain containing 3.