. 2020 Feb 10;9(2):402. doi: 10.3390/cells9020402

Table 1.

Primers used to amplify pig BimEL and BimEL mutation.

Name	Primer Sequence
BimEL	Forward	gaattcatggcaaagcaaccttccgatg
BimEL	Reverse	ggatccgcaatgtaagggggagggagggt
BimEL-S65A	Forward	ccactggccccaccgaccGCCcctggcccctttgctacc
BimEL-S65A	Reverse	GGCggtcggtggggccagtgggccctgggggctgccttg
BimEL-T112A	Forward	aaatcaacacaaGCCccaagtcctccttgccaagcc
BimEL-T112A	Reverse	acttggGGCttgtgttgatttgtcacaactcatggg
BimEL-S55^A65^A73^A	S55A-Forward	gaccgctgcccccaaggcGCCccccagggcccactg
	S55A-Reverse	GGCgccttgggggcagcggtccccttctccttccgg
	S73A-Forward	tggcccctttgctaccagaGCCccgcttttcatcttcgtg
	S73A-Reverse	GGCtctggtagcaaaggggccaggggcggtcggtgg

Mutation of BimEL-S65A and BimEL-T112A were based on pEGFP-N1-BimEL, mutation of BimEL-S55^A65^A73^A (S55A, S65A, and S73A) was based on pEGFP-N1-BimEL-S65A.