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. 2020 Jan 31;9(2):331. doi: 10.3390/cells9020331

Figure 2.

Figure 2

Inhibition of lactucin on lipid accumulation in 3T3-L1 adipocytes. (A) The molecular formula of lactucin. (B) 3T3-L1 preadipocytes were treated with the indicated concentrations of lactucin for 48 h. Cell proliferation was then determined by the CCK-8 assay (n = 5). 3T3-L1 preadipocytes were induced to differentiate with the standard regimen (IBMX, dexamethasone, and insulin; MDI) and 20 μM lactucin was added 0–6, 2–6, 4–6 and 6–8 days of adipogenesis. (C) Morphological observation and (D) Oil Red O staining of 3T3-L1 adipocytes photographed using a microscope (×100). Lipid droplets were stained in red. And absorbance of extracted ORO accumulated in lipid droplets of 3T3-L1 adipocytes was measured spectrophotometrically at 510 nm. Values are mean ± S.E.M. ** p < 0.01, *** p < 0.001.