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. 2020 Jan 31;9(2):331. doi: 10.3390/cells9020331

Figure 3.

Figure 3

The effect of lactucin on the expression of adipogenic transcription factors and adipogenic genes in 3T3-L1 adipocytes. 3T3-L1 preadipocytes were cultured in the differentiation medium containing 20 μM lactucin. (A) The mRNA levels of lipid metabolism genes in 3T3-L1 adipocytes (n = 6). (B) The protein levels of lipid metabolism genes in 3T3-L1 adipocytes (n = 4). (C) Glucose uptake as shown by 2-NBDG. (D) The effect of lactucin on the JAK2/STAT3 signaling pathway (n = 4). The immunoblot analysis of the JAK2/STAT3 signaling pathway in 3T3-L1 adipocytes (n = 4). Values are mean ± S.E.M. * p < 0.05, ** p < 0.01.