Table 5.
Primers used for sequencing and mutagenesis.
| BRAF (Exon 15) Primers for Next Generation Sequencing | ||
| BRAF (Exon 15) | FW: | TGCTTGCTCTGATAGGAAAATGA |
| RV: | TGGATCCAGACAACTGTTCAAA | |
| BRAF (Exon 15) Primers for Mutagenesis | ||
| p.G593D | FW: | GTAAAAATAGATGATTTTGGTCTAGC |
| RV: | TGTGAGGTCTTCATGAAG | |
| p.A598T | FW: | TTTTGGTCTAACTACAGTGAAATC |
| RV: | TCACCTATTTTTACTGTGAG | |
| p.V600E | FW: | CTAGCTACAGAGAAATCTCGATG |
| RV: | ACCAAAATCACCTATTTTTAC | |
| p.K601E | FW: | AGCTACAGTGGAATCTCGATG |
| RV: | AGACCAAAATCACCTATTTTTAC | |
| p.S607F | FW: | TGGAGTGGGTTCCATCAGTTT |
| RV: | TCGAGATTTCACTGTAGCTAG | |
| p.S607P | FW: | ATGGAGTGGGCCCCATCAGTT |
| RV: | CGAGATTTCACTGTAGCTAG | |
| BRAF (Exon 15) Primers for Sanger Sequencing | ||
| BRAF (Exon 15) | FW: | ACACGCCAAGTCAATCATCC |
| RV: | TCTGACTGAAAGCTGTATGGATT | |
In bold: nucleotide changes for mutagenesis.