Figure 4.

E.A./CDDP combination induced caspase activation in breast cancer cells. (A) Immunoblotting analysis of indicated proteins in 4T1 cells exposed for 48 h to the vehicle (Co) or to E.A. (6 µg/mL), CDPP (5 and 10 µM), or to the combination of E.A (6 µg/mL) and CDDP (5 or 10 µM). An anti-actin antibody (Ab) was used for loading control. One representative of three independent experiments. (B) Densitometry quantification of Western blotting obtained in (A). PARP: poly(ADP-ribose)-polymerase and c-PARP: cleaved- poly(ADP-ribose)-polymerase. The data are means ± standard deviation of three independent experiments; p-values were determined by the multiple ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001.