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. 2020 Feb 4;9(2):362. doi: 10.3390/cells9020362

Figure 6.

Figure 6

E.A./CDDP combination synergized to activate intrinsic death pathway in breast cancer cells. (A) Immunoblotting analysis of indicated proteins in 4T1 cells exposed for 48 h to the vehicle (Co) or to E.A. (6 µg/mL), CDPP (5 and 10 µM), or to the combination of E.A (6 µg/mL) and CDDP (5 or 10 µM) in the mitochondria and the cytosol. An anti-actin antibody (Ab) was used for loading control. One representative of three independent experiments. (B) Densitometry quantification of Western blotting obtained in (A). The data are means ± standard deviation of three independent experiments; p-values were determined by the multiple ANOVA test. * p < 0.05, ** p < 0.01, *** p < 0.001. Cox IV, Cytochrome c oxidase IV; HSC-70, Heat shock 70-KDa protein cognate.