Figure 2.

In vitro evaluation of EpCAM-AF680 as a NIRF imaging probe. In vitro imaging of Ishikawa^luc+ cells using BLI and EpCAM-AF680 NIRF demonstrates comparable photonic linearity (A) and increase in signal (B) with higher number of cells (range: 0–10⁶ cells). The lack of significant effects of in vitro incubation with EpCAM-AF680 antibody on proliferation and apoptosis demonstrated by MTS assay (C) and Annexin V/PI staining (D) in various cell lines. Abbreviations: Bioluminescent imaging (BLI), Epithelial cell adhesion molecule (EpCAM), Near-infrared fluorescent (NIRF), and Propium iodide (PI).