Table 1.
Antibodies. The following antibodies used in this study.
| Methods | Primary Antibody | Secondary Antibody |
|---|---|---|
| Immunogold | Rabbit anti-AQP4, 1:500, Sigma-Aldrich, A5971 | Goat anti-rabbit 15 nm, 1:20, Abcam |
| Rabbit anti-Cx30, 1:200, Invitrogen, 71-2200 | Goat anti-rabbit 15 nm, 1:20, Abcam | |
| Rabbit anti-Cx43, 1:200, Invitrogen, 71-0700 | Goat anti-rabbit 1 nm, 1:20, Abcam | |
| Rabbit anti-α-syntrophin (SYN259), 1:200, Gift from Dr Marvin E. Adams | Goat anti-rabbit 1 nm, 1:20, Abcam | |
| Western Blotting | Rabbit anti-AQP4, 1:2000, Sigma-Aldrich, A5971 | Donkey anti-rabbit HRP, 1:5000, Amersham, GE Life Sciences |
| Rabbit anti-Cx43, 1:1000, Sigma-Aldrich, C6219 | Donkey anti-rabbit HRP, 1:5000, Amersham, GE Life Sciences | |
| Rabbit anti-Cx30, 1:200, Invitrogen, 71-2200 | Donkey anti-rabbit HRP, 1:5000, Amersham, GE Life Sciences | |
| Rabbit anti-α-tubulin, 1:2000, Abcam, ab4074 | Donkey anti-rabbit HRP, 1:5000 and 1:25,000, Amersham, GE Life Sciences | |
| Custom Rabbit polyclonal anti-mouse AQP4-ex antibody, 1:2000, GeneScript [26] | Donkey anti-rabbit HRP, 1:25,000, Amersham, GE Life Sciences | |
| Custom Rabbit polyclonal anti-AQP4-M1 antibody, 1:2000, GeneScript | Donkey anti-rabbit HRP, 1:25,000, Amersham, GE Life Sciences |