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. 2020 Feb 2;9(2):344. doi: 10.3390/cells9020344

Table 1.

Optimized image thresholds and filters employed for the HCT116, FT194, and FT246 cells.

Cell Line Primary Mask Secondary Mask Spot Detection
Min OS 1 (μm) Max OS 2 (μm) DT 3 (a.u.) Ring Width (μm) Min SS 4 (μm) Max SS 5 (μm) DT 3 (a.u.)
HCT116 10 100 7000 10 1 5 3000
FT194 10 100 7000 15 1 6 3000
FT246 10 100 7000 15 1 6 3000

1 Minimum object size; 2 Maximum object size; 3 Detection threshold (arbitrary units); 4 Minimum spot size; 5 Maximum spot size.