Figure 2.

Proliferation and DNA repair-relevant gene promoters are controlled by BRG1 and EP300 activity in breast cancer cells. (A and C) Long-term (48 h) deficiency of BRG1 and EP300 activity limits transcription of genes encoding components of cell division and DNA repair mechanisms. The data present Log2FC of mRNA level measured by real-time PCR normalized to untreated control (iEP300 and iSWI/SNF) and non-template control (siBRG1). (B and D) Gene repression results in substantial loss of proliferation markers as seen from western blot, and by cell growth arrest in G1 phase (E). (F) Inhibition of BRG1 and EP300 similarly decreases the amount of proteins involved in removal of DNA damage. Heatmaps with long-term BRG1 and Ep300 deficiency (A and C) were prepared from four independent replicates for iEp300, and iSWI/SNF; from 3 for siBRG1. Detailed statistical analysis is presented in supplementary Table S3. Western blots in B, D, and F show representative images from three independent experiments. Pictures show cropped areas of western blots. Whole images are included in Figure S3. Analysis of the cell cycle was carried out twice in two technical replicates for each condition.