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. 2020 Feb 3;9(2):356. doi: 10.3390/cells9020356

Figure 1.

Figure 1

Effects of transforming growth factor-β1 (TGF-β1) on cell morphology and epithelial-to-mesenchymal transition (EMT) markers. (A) Morphological changes of BEAS-2B cells after stimulated with TGF-β1 (3 ng/mL) for 24 h. Spindle-like cells were indicated with red arrow. (B–D) Gene (B) and protein (C,D) expressions of E-cadherin, collagen Ӏ, fibronectin and α-smooth muscle actin (α-SMA) were analyzed in BEAS-2B cells with or without TGF-β1 stimulation using real-time quantitative PCR and western blotting, respectively. Representative western blotting images of E-cadherin, collagen Ӏ, fibronectin and α-SMA were shown in (C). (E) Immunofluorescence images of ZO-1, E-cadherin, and collagen Ӏ after 24 h stimulation of TGF-β1. Scale bar represents 100 µm. Data represent 3–6 independent experiments. Data are expressed as mean ± SEM, * p < 0.05; significant difference between indicated groups.