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. 2020 Feb 3;9(2):356. doi: 10.3390/cells9020356

Figure 6.

Figure 6

Role of AKAPs—Ezrin, AKAP95, and Yotiao—in TGF-β1-induced cell migration using BEAS-2B cells. (A) Representative images of wound healing assay of BEAS-2B cells after 24 h post scratch. The white dotted line indicated borders of scratches at 0 h. (B) Quantification of wound closure of cells pre-incubated with 50 µM st-Ht31 for 30 min., following stimulated with 3 ng/mL TGF-β1 for 24 h. (C) Representative images of wound healing assay of BEAS-2B cells after 24 h post scratch. The white dotted line indicated borders of scratches at 0 h. (D) Quantification of wound closure of TGF-β1 treated cells icombined knockdown of Ezrin, AKAP95, and Yotiao. (E) 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in BEAS-2B cells that were transfected with a combination siRNA of Ezrin, AKAP95, and Yotiao together with 3 ng/mL TGF-β1 and 1% CSE exposure. (F) Cell cycle distribution in BEAS-2B cells that were transfected with a combination siRNA of Ezrin, AKAP95, and Yotiao together with 3 ng/mL TGF-β1 and 1% CSE exposure. The data represent 3–5 independent experiments. Data are expressed as mean ± SEM, * p < 0.05; significant difference between indicated groups.