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. 2020 Feb 3;9(2):356. doi: 10.3390/cells9020356

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of dbcAMP on TGF-β1-induced EMT markers expression in BEAS-2B cells. (A,B) protein expressions of E-cadherin (A) and collagen Ӏ (B) were analyzed in BEAS-2B cells pre-incubated with 10 μM or 100 μM dbcAMP for 30 min. before TGF-β1 stimulation. (C,D) Immunofluorescence images (C) and quantification (D) of ZO-1 and collagen Ӏ after TGF-β1 stimulation for 24 h. Scale bar represents 100µm. Data represent 4-6 independent experiments. Data are expressed as mean ± SEM, * p < 0.05; significant difference between indicated groups; # p < 0.05; significant difference between with or without TGF-β1 treatment.