Figure 5.

PA-RL displays a selective cytotoxic effect on Epithelial Ovarian Cancer (EOC) cell lines. (a) Viability of SKOV-3 (n = 7) and OV-90 (n = 9) cell lines treated with PA-RL dilutions (1:4, 1:8 and 1:16). Data are mean ± SEM normalized on the corresponding control in RL (CTR-RL). (b) Viability of SKOV-3 and OV-90 cell lines treated with PA-RL 1:16 and synthetic solutions at dilution 1:16. H₂O₂-supplemented RL, NO₂⁻-supplemented RL and pH-adjusted RL solutions were diluted in RL to obtain the final treatment solutions. Data are mean ± SEM (n = 3) normalized on the corresponding CTR-RL. (c) Viability of non-cancer cells, namely human fibroblasts (n = 9) and HOSE (n = 4) treated with different PA-RL dilutions (1:4, 1:8 and 1:16). Data are mean ± SEM normalized on the corresponding CTR-RL. (d) PA-RL 1:16 efficacy on cell viability in non-cancer and EOC cell lines. Cell viability was normalized to the CTR-RL at 2 h and plotted as percentage relative to the corresponding CTR-RL, for both time points. In each panel, data are mean ± SEM and statistical significance is specified with asterisks (* p ≤ 0.05, ** p ≤ 0.001 as determined by a paired Student’s t-test).