Skip to main content
. 2020 Feb 6;9(2):379. doi: 10.3390/cells9020379

Figure 6.

Figure 6

Effect of Fraction D in leukemia and normal cell lines. Cell growth rates determined by MTT of (A) U937 and (B) NB4 cells treated with Fraction D for 24 and 48 h, at the indicated concentrations, and of (D) Mepr2B cells challenged with Fraction D for 48 h, at the indicated concentrations. (C) STAT5 gene expression analysis of U937, NB4, and K562 cells treated with Fraction D at 0.1 mg/mL, for 24 and 48 h. Values are mean ± SD of biological triplicates. (E) pY177AB, pY694STAT5, pY308AKT, AKT, and pT180/Y182p38 expression levels in Mepr2B cells treated with Fraction D at 0.1 mg/mL for 24 and 48 h. (F) Normalization of protein level expression by the ImageJ software. Values are mean ± SD of biological duplicates. **** p-value ≤ 0.0001, *** p-value ≤ 0.001, ** p-value ≤ 0.01, * p-value ≤ 0.05, ns p-value > 0.05 vs. control cells.