Figure 2.
Effect of Lactobacillus casei administration on the ex vivo cytokine production. BALB/c mice received daily 109 CFU of live L. casei for 13 days (n = 4/5). Control group received PBS (n = 4/5). On day ten (10) of oral administration, 5 × 106 CT26 cells were injected subcutaneously. PP were excised after euthanasia three (d13 from start) or seven (d17 frorm start) days post-inoculation of CT26 cells. Cytokine levels of (a) IFN-γ, (b) IL-12 and (c) IL-10 were quantified by ELISA in a PP lysate of L. casei (LC)-fed mice as compared to the respective lysate of PBS-fed control mice. Cytokine production was determined at the indicated time points by ELISA following the manufacturer’s instructions. *, p < 0.05, groups were repeated two times.