Skip to main content
. 2020 Jan 25;9(2):290. doi: 10.3390/cells9020290

Figure 1.

Figure 1

Characterization of primary mouse HSC. (A) Primary D4 or P1 mHSC were evaluated for vitamin A autofluorescence (left) or examined by phase contrast (right), and imaged by confocal microscopy. Based on six fields, Vitamin A was positive in 91.3 ± 3.4% of D4 mHSC and 85.4 ± 8.0% of P1 mHSC. (B) D4 or P1 mHSC were stained with anti-αSMA (left) which was then merged with DAPI (right). Based on three fields, α SMA was positive in 3.3 ± 0.7% of D4 mHSC and 93.3 ± 5.0% of P1 mHSC. (C) D4 or P1 mHSC were stained with anti-reelin (left) which was then merged with DAP1 (right). Based on two fields, the frequency of reelin-positive cells was 98.4 ± 1.6% for D4 mHSC and 13.1 ± 1.2% for P1 mHSC. Bars: 50 µm for A and C; 100 µm for B.