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. 2020 Feb 13;9(2):438. doi: 10.3390/cells9020438

Figure 2.

Figure 2

Histological and immunohistochemical investigation of the autoimmune retinopathy (AIR) and control donors. (A–D). Hematoxylin and eosin staining of the AIR and control donors. Sections from the periphery of a control donor (donor 2) (A), the macula (OD) of the AIR donor (B), and the periphery of the AIR donor (C,D). The AIR macula demonstrates intact ganglion cell and inner nuclear layers with attenuated cone photoreceptor outer segments. In contrast, in the periphery of the AIR donor complete loss of the outer nuclear layer (C) and retinal pigment epithelium (RPE) pigment migration into the inner retina (D) is observed (*). (E,F): Cone opsins (blue cone opsin and red/green cone opsins) are labeled in red while RetP1 is labeled in green. (E): A macula from a donor with normal ocular history demonstrates abundant labeling of cone opsins and rhodopsin. Of note, the RPE below the photoreceptors is out of frame. (F) The macula from the AIR donor demonstrates a complete lack of rod photoreceptors with rare, extremely attenuated cone photoreceptors (arrows). Autofluorescent lipofuscin from the RPE appears below the photoreceptor cells. Scalebar (100 microns) for all subpanels is provided in (E).