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. 2020 Feb 13;9(2):438. doi: 10.3390/cells9020438

Figure 3.

Figure 3

Single-cell RNA sequencing of the AIR donor. (A,B): Five human donor eyes were used for this study. A gross image of a control eye (donor 4) (A) and the AIR eye (donor 5) (B) are included. From each eye, a 2 mm foveal centered punch (red) and an 8 mm peripheral punch isolated from the inferotemporal region (blue) were acquired and gently dissociated. Scalebar (A) is 5 mm. (C): Single-cell RNA sequencing of retinal cells from the AIR donor and four control patients. A total of 23,429 cells were recovered after filtering. Unsupervised clustering of cells resulted in 23 clusters, which are visualized with uniform manifold approximation and projection (UMAP) dimensionality reduction, where each point represents the multidimensional transcriptome of a single-cell and each cluster of cells is depicted in a different color. (D): Violin plots depict the expression of cell-type specific genes across the 23 identified clusters. Per = peripheral retina. AIR = autoimmune retinopathy. RPE = retinal pigment epithelium. RGC = retinal ganglion cell.