Figure 4.

Library composition of recovered cells. (A): In the fovea, cone photoreceptor cells synapse with one bipolar cell, which synapse with one retinal ganglion cell. (B): The proportion of each cell type recovered from the fovea of the four control donors and the autoimmune retinopathy donor. No foveal rods were recovered from the AIR donor. (C): In order to visualize the degree of gene expression differences within each population of cells between the AIR and control donors, differential expression analysis was performed. In each cell type, the number of differentially expressed genes that were enriched in the AIR donor and the control donors were enumerated and divided by the total number of expressed genes (in at least 10% of cells). For example, 1.09% of foveal Müller cell genes were significantly enriched in the AIR donor (dark grey), while 0.57% of foveal Müller cell genes were significantly enriched in the control donors (light grey). (D): In the periphery, multiple rod photoreceptor cells synapse with a single bipolar cell. (E): The proportion of each cell type recovered from the periphery of the four control donors and the periphery of the AIR donor. (F): As in (C), the proportion of differentially expressed genes between the AIR and control donors was performed in each cell type. More genes were differentially expressed in the periphery compared to the fovea (C). As no RPE cells originated from control donors, differential expression could not be performed in the periphery for this cell type.