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. 2020 Jan 22;12(2):268. doi: 10.3390/cancers12020268

Figure 6.

Figure 6

Effects of PEITC on stemness in MDA-MB-231/IR cells. (a) Mammospheres were cultured in complete MammoCult Human Medium (100× magnification). (b) CD44+/CD24− populations were assessed by FACS analysis after incubation with PEITC (0, 5, 10, 15, or 20 μM) for 24 h. (c) The ALDH+ population was examined using the ALDEFLUOR assay kit, with DEAB used as a negative control; cells were treated with PEITC (0 or 5 μM) for 24 h. (d) Cell migration was determined by the wound healing assay; cells were treated with PEITC (0 or 5 µM) for 24 or 48 h (100× magnification). (e) Invasive cells were stained with crystal violet after treatment with PEITC (0 or 5 µM) for 24 h (100× magnification). (f) Western blot analysis of Slug after incubation of cells with PEITC (0, 1.25, 2.5, or 5 μM) for 24 h; * p < 0.05; results are presented as mean ± standard deviation.