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. 2020 Feb 12;10(2):283. doi: 10.3390/biom10020283

Figure 5.

Figure 5

A549 lung cancer cell line viability inhibition by acylcholines. The cells were incubated with the substances for 24 h. Cell viability was determined using an MTT test. (A) the cytotoxicity of acylcholines. In the ANOVA with the Tukey post-hoc test, the survival of the cells at the Ol-CHOL concentration 100 μM differed statistically significant from other acylcholines with p < 0.05. Mean ± standard deviation, N = 3 experiments; (B) Apoptosis induction by 150 μM of Ol-CHOL, green phosphatidylserine sensor fluorescence, red membrane-impermeable nucleus stain 7-AAD, fluorescence microscopy, scale bar is 100 μm; (C) Caspase activation after 5 h incubation with either 100 μM of N-docosahexaenoyl dopamine (DHA-DA, positive control) or 150 μM of Ol-CHOL with or without 100 μM of the pan-caspase inhibitor Z-VAD-FMK, fluorescence of hydrolyzed specific substrates Ac-DEVD-AFC (Caspase 3), Ac-LEHD-AFC (Caspase 9), and SCP0139 (Caspase 8). Mean ± standard deviation, N = 3 experiments. *, a statistically significant difference from the untreated control; (D) the cytotoxicity of Ol-CHOL in the presence of nicotine. Mean ± standard deviation, N = 3 experiments.