Table 1.
The results of 125I-αBgt competition assay for acylcholines. The IC50 values presented (95% CIs). In the ANOVA with Holm–Sidak post-test, the differences between all acylcholines on human a7 nAChR were significant with p < 0.05 except for the pairs Ln-CHOL vs. Ol-CHOL and Ol-CHOL vs. ACh; on T. californica nAChR, the differences between all acylcholines on human a7 nAChR were significant with p < 0.05 except for the AA-CHOL and Ol-CHOL pair; on AChBP, only differences in pairs AA-CHOL vs. Ln-CHOL, Ln-CHOL vs. Ol-CHOL, Ln-CHOL vs. DHA-CHOL, Ln-CHOL vs. ACh, and Ol-CHOL vs. DHA-CHOL were significant with p < 0.05.
| Ligand |
IC50, μM
(95% CI) |
||
| L. stagnalis AChBP | T. californica nAChR | human α7 nAChR | |
| AA-CHOL | 21.3 (17.1–26.5) |
47.6 (34.9–58.1) |
35.9 (31.3–41.2) |
| Ln-CHOL | 2.3 (2.10–2.65) |
18.7 (16.1–21.9) |
23.1 (18.2–25.9) |
| Ol-CHOL | 13.4 (11.5–15.2) |
39.8 (37.4–42.3) |
14.2 (11.7–17.3) |
| DHA-CHOL | 25.1 (22.1–28.5) |
~ 93 | ~ 80 |
| ACh | 20.1 (15.8–25.5) |
7.6 (5.97–9.33) |
4.29 (2.77–6.67) |