Figure 8.

Spheroid formation capability of FTC-133 cells cultured on the RPM. (A) Comparison of transcription regulation patterns 4 h after DEX supplementation (yellow bars) and after a three-day RPM-exposure (grey bars). Bold gene symbols indicate fold changes >2.5 or regulation in opposite directions. (B) Cells 24 h after the RPM-experiment started; (C) Cells 24 h after an initial two-day RPM exposure. Medium was refreshed and spheroids were discarded after the first two days. Although many genes were similarly regulated after DEX supplementation and after a three-day-exposure to the RPM, in contrast to DEX treatment, cells did not lose the ability to form spheroids in µg. Scale bars: 100 µm.