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View full-text article in PMC

. 2020 Feb 7;12(2):385. doi: 10.3390/cancers12020385

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of LAN-1 cells recovered from a xenograft primary tumor (LAN-1-PT) and corresponding spontaneous bone metastasis (LAN-1-BM). (A) F-actin immunocytostaining of LAN-1-PT and LAN-1-BM cells analyzed for length, width, and number of filopodia-like protrusions per cell. (B) Cell transmigration through a porous membrane was similar between both sublines, while the relative invasive potential was by trend higher in the LAN-1-BM subline. (C) LAN-1-BM cells showed a strong increase in vimentin expression. (D) Cell viability (MTS assay) and proliferation was decreased in the LAN-1-BM subline. (E) Colony formation assays in soft agar showed decreased numbers and diameters of tumor spheres formed by the LAN-1-BM subline. (F,G) After re-injection and a second round of re-cultivation and re-injection (of the respective sublines LAN-1-PT² and LAN-1-BM²), the pre- and post-surgical survival periods and tumor weights at surgery were quite comparable between the PT/BM and PT²/BM² groups. (H) Unexpectedly, there was no increase, but rather a decrease in the incidence of spontaneous BM metastases in the LAN-1-BM/BM² as compared to the LAN-1-PT/PT² groups. * p < 0.05, ** p < 0.01, *** p < 0.001.