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. 2020 Feb 14;9(2):443. doi: 10.3390/cells9020443

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Energy metabolism in intact KI cells. (A) Cellular oxygen consumption rate (OCR) and (B) extracellular acidification rate (ECAR) were recorded by an XFe Bioenergetics Analyzer (Seahorse) with endogenous substrates (basal) and after subsequent additions of 10 mM glucose (Glu), 1 µM oligomycin (Oligo), 1 µM FCCP (FCCP) in HEK293 cells (blue, n = 5), COX4i1 KI (green, n = 7, at least two replicates of each of three clones), and COX4i2 KI cells (magenta, n = 8, at least two replicates of each of three clones), plotted as mean values ± S.D. Glycolytic reserve capacity displayed in B was calculated as the difference between the ECAR values in Oligo and Glu states. (C) OCR/ECAR ratio (mean ± S.D.) was calculated from recorded rates in the coupled state in the presence of 10 mM glucose (Glu). * p < 0.05 marks a significant difference between COX4i1 and COX4i2 KI cells.