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. 2020 Feb 12;9(2):431. doi: 10.3390/cells9020431

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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T-regulatory cells in mixed lymphocyte reaction MLR with MSCs of patients with non-malignant hematological diseases and healthy donors. (A) Dotplot of CD3+CD45+ T cells which were determined by flow cytometry after staining of mononuclear cells with anti-CD45/CD3/CD4/CD25 antibodies on day 0 in MLR. (B) Percentage of CD24+CD25+ cells after gating on CD45+CD3+ lymphocytes on day 0 and 7 (C). (D) The percentage of T-regs in each study group on day 7 of MLR. HD: healthy donors; SCD: sickle cell disease; TM: thalassemia major; SCN: severe congenital neutropenia.