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. 2020 Feb 18;9(2):459. doi: 10.3390/cells9020459

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of depolarizing current injections on the membrane potential of TRPC1^+/+ and TRPC1^−/− neurons. (a) Response typical for neurons of both genotypes before DHPG application. Data from a TRPC1^−/− neuron. Current injection: +20 pA. (b–d) Examples of activity patterns observed in response to current injection in the presence of DHPG. (b) A TRPC1^+/+ neuron that showed no afterpotential (nAP). (c) A TRPC1^+/+ neuron that showed a long plateau potential (LPP). (d) A TRPC1^−/− neuron that showed a short plateau potential (SPP). The insets show the beginning of the reponse on an expanded time scale. The scale bar in the inset represents 0.2 s. Neurons were kept at potentials close to −60 mV by current injection. The symbols under the potential traces in (a–d) indicate the times at which the potentials in (e, f) were measured. (e, f) Potential before and after the depolarizing current injection in the absence (basal) and presence of DHPG (DHPG) in TRPC1^+/+ (e) and TRPC1^−/− (f) neurons. (g) Percentage of cells showing each response type in DHPG. (h) Duration of plateau potentials in response to current injection in DHPG in TRPC1^+/+ (filled bars) and TRPC1^−/− neurons (open bars). Numbers on the columns in (g, h) indicate the number of neurons. Dots indicate the values from individual neurons.