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. 2020 Feb 24;9(2):511. doi: 10.3390/cells9020511

Figure 6.

Figure 6

The influence of (R)-salbutamol on Th17 cells and Treg cells levels. Spleen cells were obtained from mice on day 8 and then stimulated with cocktail (with Brefeldin) for 6 h. Thereafter, they were stained with fluorescent conjugated anti-mouse CD3, CD25, and CD4. In addition, intracellular staining of IL-17 and Foxp3 was performed using the respective antibodies. Representative contour plots showed the frequency of live CD4+ T cells, IL-17+ Th17 gated and CD25+ Foxp3+ Treg in the splenocytes isolated from mice treated with IMQ and then with (R)-salbutamol. Relative scatter plots showed the frequencies determined from live cells. (A) Representative dot plots showing the percentage of CD3+CD4+ T cells. (B) Statistical analysis of the percentage of CD3+CD4+ T cells. (C) Expression of intracellular cytokines IL-17 was detected by flow cytometry in cells gated for CD4+. (D) FoxP3 stained with a Foxp3 staining buffer set without stimulation, with CD25+ surface as the gate. (E,F) Statistical analysis of the above results. ## p < 0.01, relative to IMQ group. ** p < 0.01, relative to the control group. Error bars represent the mean ± SD (n = 8).