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. 2020 Feb 28;9(2):465. doi: 10.3390/cells9020465

Figure 4.

Figure 4

V-ATPase inhibition increases HIF-1α stabilization and AMPK activity and a similar trend is seen after a3 KD. Panc-1 and BxPC-3 cells were treated with either DMSO ctrl., 2 or 10 nM ConA for 48 h (A–C,G,H), or were transfected with Mock siRNA or 2 different siRNAs targeting a3, and grown for 48h (D–F,I–K), followed by Western blot analysis. (A,G and D,I) shows representative blots of HIF-1α, AMPK, and pAMPK in cells treated with ConA (n = 3–6) or in cells transfected with a3 siRNAs (n = 3–8). B,E,F,H,J,K) show quantification of the protein levels of HIF-1α (B,C,E,F) and pAMPK/AMPK (G–K) in cells treated with ConA or siRNA targeting a3. Data was normalized to beta-actin or GAPDH (loading ctrl.) and the level in respective DMSO or Mock ctrl. Data is shown as mean with S.E.M. error bars, of 3–6 independent experiments per cell line and siRNA. Rel. = relative.