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. 2020 Feb 19;9(2):481. doi: 10.3390/cells9020481

Figure 2.

Figure 2

R-Tf-D-LP4 peptide treatment reduces the levels of blood glucose in ob/ob mice and decreases fat accumulation in 3T3-F442A adipocytes. (A) ob/ob mice were untreated (●) or treated (▲) with intravenous injections of the R-Tf-D-LP4 peptide (14 mg/kg) three times a week, and blood was drawn from an incision in the tail to measure glucose levels. The data represent the means ± SD (n = 6 mice). *p < 0.05. The dashed red line represents the blood glucose levels of WT mice. (B) Body weight of ob/ob mice treated with the R-Tf-D-LP4 peptide (14 mg/kg) as followed for 7 weeks. (C) The change in body weight as a function of time is presented. (D) 3T3- F442A cells were incubated with the indicated concentration of the R-Tf-D-LP4 peptide for 24 h and then stained with Oil Red O and photographed to visualize the lipid droplets stained in red. (E) Quantification of Oil Red O stain was carried out by stain extraction with 100% isopropanol for 5 min and measurement of the absorbance at 492 nm. The results are means ± SEM (n = 3; *** p ≤ 0.001).