Figure 4.

R-Tf-D-LP4 effect on the Langerhans islets in STZ/HFD-32 fed mice. (A) Representative paraffin-embedded, formaldehyde-fixed pancreas sections from chow-fed, or STZ/HFD-32 fed mice with and without R-Tf-D-LP4 peptide treatment. At the steatosis stage, sections were IF stained with anti-insulin antibodies, and the nuclei were stained with DAPI. White arrows point to disrupted islets, and yellow arrows point to small non-disrupted ones. (B) Pancreatic sections from chow-fed, STZ/HFD-32 fed mice with and without R-Tf-D-LP4 peptide treatment at the NASH stage were stained with H&E. These islets are circled.