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ob/ob mice show large islets where their number was increased by R-Tf-D-LP4 peptide treatment. ob/ob mice were treated or not treated with intravenous injections of the R-Tf-D-LP4 peptide (14 mg/kg) three times a week, mice were sacrificed, and the pancreases were removed and fixed. Representative paraffin-embedded, formaldehyde-fixed pancreatic sections were stained with Hematoxylin, eosin (A), or IF stained with anti-insulin antibodies, and the nuclei were stained with DAPI (B).
