Table 2.

Effect of R-Tf-D-LP4 peptide treatment on the number and size of Langerhans islets in STZ/HFD-32 diet-fed mice with steatosis or NASH. Insulin-stained Langerhans islets were counted, measured, and categorized according to their size. The number of total islets counted per pancreas is presented. ns indicates not-significant.

	Steatosis
Parameter	Chow	HFD-32	HFD-32, Peptide
Total no. of islets	136 (n = 5)	217 (n = 16)	451 (n = 17)
Average no. of islets per mouse	27	14 (<0.001)	27 (ns)
Islet size, 1–50 mm	11	4 (<0.05)	16 (ns)
Islet size, 51–100 mm	8	6 (<0.05)	8 (ns)
Islet size, 101–150 mm	5 (ns)	3 (ns)	2 (ns)
Islet size, 151–300 mm	3	1 (<0.05)	1 (ns)
	NASH
Parameter	Chow	HFD-32	HFD-32, peptide
Total no. of islets	136 (n = 5)	168 (n = 10)	354 (n = 10)
Average no. of islets per mouse	27	17 (<0.001)	35 (<0.05)
Islet size, 1–50 μm	11	8 (ns)	16 (ns)
Islet size, 51–100 μm	8	6 (ns)	12 (ns)
Islet size, 101–150 μm	5	2 (ns)	4 (ns)
Islet size, 151–350 μm	3	1 (ns)	3 (ns)