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. 2020 Feb 21;9(2):496. doi: 10.3390/cells9020496

Figure 5.

Figure 5

The catalytic activity of eNAMPT is dispensable for CCR5 antagonism. (A) Left, representative calcium traces of HeLa-CCR5 loaded with FURA-2AM and treated with RANTES (25 ng/mL; 3 nM) alone or pretreated with eNAMPT/eNAMPT H247E (500 ng/mL; 9 nM) for 100 s. Right, histograms of percentage of AUC (middle panel) and responding cells (right panel) as mean ± S.E.M. (180–210 cells from four independent experiments). (B) Left, representative calcium traces of HeLa-CCR5 loaded with FURA-2AM and pretreated for 100 s with vehicle or NMN (100 µM) before the addition of Rantes (25 ng/mL = 3 nM). Histograms of percentage of AUC (right panel) and responding cells (left panel) as mean ± S.E.M. (99–126 cells from three independent experiments). * p < 0.05, ** p < 0.01.