Table 1.
Immunohistochemistry (IHC) parameters.
| Antibody | Vendor a | Catalog No. | Species | Clone | Pretreatment | Dilution |
|---|---|---|---|---|---|---|
| Claudin-1 | a | 51-9000 | Rabbit polyclonal | n.a. | High pH | 1:200 |
| E-cadherin | b | 790-4497 | Mouse monoclonal | 36 | High pH | Ready-to-use |
| Ki67 | b | 790-4286 | Rabbit monoclonal | 30-9 | High pH | Ready-to-use |
| P53 | b | 760-2542 | Mouse monoclonal | Bp53-11 | High pH | Ready-to-use |
| SNAIL/SLUG | c | ab224731 | Mouse monoclonal | CL3700 | High pH | 1:50 |
| Vimentin | b | 790-2917 | Mouse monoclonal | V9 | High pH | Ready-to-use |
| ZEB1 | c | ab203829 | Rabbit monoclonal | EPR17375 | Low pH | 1:50 |
| ZEB2 | c | ab230561 | Rabbit monoclonal | EPR21246 | Low pH | 1:500 |
a Vendors: a = ThermoFisher (Rockford, IL, USA), b = Ventana (Tucson, AZ, USA), c = Abcam (Cambridge, UK).