Table 3.
K-means cluster analysis of EMT and proliferation markers.
| Factor | IHC Mean Scores | p | |
|---|---|---|---|
| Cluster | |||
| 1 | 2 | ||
| Vimentin score (center) | 20.17 | 2.57 | 0.002 |
| Vimentin score (margin) | 33.00 | 6.40 | <0.001 |
| E-cadherin score (center) | 126.83 | 269.00 | <0.001 |
| E-cadherin score (margin) | 102.33 | 242.67 | <0.001 |
| Claudin-1 (center) | 107.17 | 207.67 | 0.001 |
| Claudin-1 (margin) | 52.67 | 182.67 | <0.001 |
| p53 (center) | 170.67 | 122.50 | 0.370 |
| p53 (margin) | 158.03 | 131.40 | 0.628 |
| Ki67 (center) | 69.67 | 41.87 | 0.009 |
| Ki67 (margin) ZEB1 (center) |
43.00 | 43.10 | 0.993 |
| 0.40 | 0.23 | 0.396 | |
| ZEB1 (margin) | 1.60 | 0.57 | 0.029 |
| ZEB2 (center) | 1.33 | 0.70 | 0.283 |
| ZEB2 (margin) | 3.90 | 1.90 | 0.135 |
| Snail/Slug cytoplasmic (center) | 149.67 | 135.33 | 0.569 |
| Snail/Slug cytoplasmic (margin) | 113.67 | 131.83 | 0.404 |
| Slug/Snail nuclear (center) | 39.83 | 8.47 | 0.003 |
| Slug/Snail nuclear (margin) | 77.17 | 13.67 | 0.001 |
| miR-141 | 0.194 | 0.226 | 0.662 |
| miR-200a | 0.517 | 0.436 | 0.662 |
| miR-200b | 5.154 | 2.869 | 0.293 |
| miR-200c | 1.390 | 0.867 | 0.298 |
| miR-205 | 0.310 | 1.498 | 0.040 |
| miR-429 | 0.285 | 0.375 | 0.494 |
IHC mean scores represent calculated cut-off values to distinguish the two clusters. Green and light green highlighted boxes indicate highly significant and significant discrimination factors, respectively.