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. 2020 Feb 11;21(4):1206. doi: 10.3390/ijms21041206

Table 1.

Oligonucleotides used for qRT-PCR analysis, for construction of monocistronic and dicistronic vectors, and for the evaluation of cryptic splicing.

Primer Oligonucleotide Sequence
ACLY-RTFor 5′-TACATCTGCAAAGTGAAGTGG-3′
ACLY-RTRev 5′-TTCAGCAAGGTCAGCTTCAG-3′
SREBP-1For 5′-ACACCATGGGGAAGCACAC-3′
SREBP-1Rev 5′-CTTCACTCTCAATGCGCC-3′
rRNA18SFor 5′-GTTGGTTTTCGGAACTGAGGC-3′
rRNA18SRev 5′-CGGTCGGCATCGTTTATGGT-3′
ACLY For1-pRF/pHpRF 5′-AAGCTTGAATTCAGCCGATGGGGGCGGGGAAA-3′
ACLY Rev1-pRF/pHpRF 5′-GAATTCCATGGCTGCAGAGAGACCTGCTC-3′
ACLY For1-pGL3prom 5′-GAATTCAAGCTTAGCCGATGGGGGCGGGGAAA-3′
ACLY Rev1-pGL3prom 5′-GAATTCCATGGCTGCAGAGAGACCTGCTC-3′
CSFor 5′-GGCTTCCAAGGTGTACGACCCCGAG-3′
CSRev 5′-GGGCCCTTCTTAATGTTCTTAGCAT-3′